nt final concentration of 20 nm  (New England Biolabs)

Journal: Nature protocols

Article Title: A Whole-Tissue RNA-seq Toolkit for Organism-Wide Studies of Gene Expression with PME-seq

doi: 10.1038/s41596-019-0291-y

Figure Lengend Snippet:

Article Snippet: Add the following components to each sample: table ft1 table-wrap mode="anchored" t5 Component Volume (μl) Final concentration NEBuffer 2 (10x) 2 1X dNTPs (100 mM) 0.8 4 mM Klenow Fragment (3’- 5’ exo-) 1 (5U/μl) 0.25U/μl PEG 8000 50% (wt/vol) 10 25% Total 13.8 Open in a separate window CRITICAL STEP: PEG 8000 50% (wt/vol) is viscous and must be added carefully.

Techniques: Concentration Assay